Physiological and Genetic Responses of Resistant and Susceptible spring wheat in grain-filling stage of development to water limitation - Essay Prowess

Physiological and Genetic Responses of Resistant and Susceptible spring wheat in grain-filling stage of development to water limitation

Physiological and Genetic Responses of Resistant and Susceptible spring wheat in grain-filling stage of development to water limitation

Title:  Physiological and Genetic Responses of Resistant and Susceptible spring wheat in grain-filling stage of development to water limitation

 

Introduction: (3pages)

 Wheat importance to world agriculture

The importance of wheat growth and development under water limiting conditions

Evidence for resistance to water limitations among spring wheats in re-productivity stage

Background about the importance of drought tolerance genes.

How do plants respond to water limitations genetically

Effect of drought on biomass weight, spikes length, spikes weight, seeds number in spikes, and seeds weight.

Limitations of previous studies on drought stress

Literature review: (7 pages)

-The last reviewed paper.

5 pages for RNA sequencing

2 pages for Physiological effects.

Objective and objective significance: (1 page)

The objective:

1-      Transcriptomic analysis for spikes and flag leaf of two varieties of spring   wheat. Alpowa (resistance) and Idaho (susceptible)

2-      Analysis for Physiological effects for the study varieties.    

Methods Objective: (5 pages)

1-      Materials and methods (I already wrote this part, and the experiment about three levels of water on two spring wheat varieties during grain filling.  [Alpowa (resistance) and Idaho (susceptible)]

RNA extraction and synthesis of cDNA

Trizol reagent protocol

DNA quantification

RNA Seq library formation

HiSeq Sequencing

            Illumina library preparation

            Illumina hiseq 2500

Quality filtering of sequences

            Quality filtering Q30

Sequence alignments

            Alignment unique gene

Differential Gene Expression Analysis

CUFF links program identify

Statistical Analysis by using R program and R packages

            Gene extraction analysis

            Functional analysis (functional analysis using DAVID bioinformatics)

Expected results.