and Genetic Responses of Resistant and Susceptible spring wheat in
grain-filling stage of development to water limitation
Wheat importance to world agriculture
importance of wheat growth and development under water limiting conditions
for resistance to water limitations among spring wheats in re-productivity
about the importance of drought tolerance genes.
do plants respond to water limitations genetically
of drought on biomass weight, spikes length, spikes weight, seeds number in spikes,
and seeds weight.
of previous studies on drought stress
Literature review: (7 pages)
-The last reviewed paper.
5 pages for RNA sequencing
2 pages for Physiological effects.
Objective and objective significance: (1 page)
Transcriptomic analysis for spikes and flag leaf of
two varieties of spring wheat. Alpowa
(resistance) and Idaho (susceptible)
Analysis for Physiological effects for the study
Methods Objective: (5 pages)
Materials and methods (I already wrote this part,
and the experiment about three levels of water on two spring wheat varieties
during grain filling. [Alpowa
(resistance) and Idaho (susceptible)]
extraction and synthesis of cDNA
RNA Seq library formation
Quality filtering of sequences
Differential Gene Expression Analysis
CUFF links program identify
Statistical Analysis by using R program and R
analysis (functional analysis using DAVID bioinformatics)